Cleverson D Souza
Assistant Professor, Diplomate ACVP
Office Phone: 509-335-0726
Dr. Souza is a dual citizen of Brazil and Italy and received his Doctor of Veterinary Medicine (DVM) in 1998 from the Universidade Federal do Parana, PR, Brazil. Dr Souza has been trained in research and pathology at multiple Universities. After receiving his DVM he completed a 2 year residency program in Sao Paulo/Br followed by a thesis Master Science degree in Clinical Pathology at Universidade Federal de Santa Maria/Rio Grande do Sul/Br. He worked as a veterinary clinical pathologist at Universidade de Passo Fundo/RS for 1 year before accepting a PhD position and joining Dr Douglas J Weiss (DVM, PhD, DACVP) lab at the University of Minnesota/USA. The research involved unraveling key cell signaling pathways important for the ability of Mycobacterium paratuberculosis to survive within macrophages. This training was followed by a residency in veterinary clinical pathology at University of Minnesota/USA. He then successfully completed his specialty board certification through the American College of Veterinary Pathologists (ACVP). From 2009 to 2012, Dr Souza worked at Universidade de Fortaleza, UniChristus and Universidade Federal do Ceara, all in Brazil, where he was involved with the instruction of general pathology to MDs and DDs students. Dr Souza is currently a tenured-track faculty at Washington State University where he work as a clinical pathologist (service, teaching and research) at the Veterinary Clinical Science Department.
- Ph.D - University of Minnesota, St Paul, MN. Project: Host-pathogen interaction in Mycobacterium avium subsp. paratuberculosis infection: Role of the mitogen activated protein kinases in regulating antimicrobial responses of mononuclear phagocytes
- Residency in Veterinary Clinical Pathology - University of Minnesota, MN, USA
- MSc – Universidade Federal de Santa Maria (Immunology track) RS, Brazil. Project: Evaluation of the neutrophilic oxidative burst in Saanen goats naturally infected by Haemonchus contortus
- Residency in Veterinary Clinical Pathology - Universidade Estadual Paulista/UNESP, SP, Brazil
- DVM – Universidade Federal do Parana, PR, Brazil
Dr. Souza’s lab investigate the cell signaling pathways modulated upon Mycobacterium avium subsp paratuberculosis (MAP) early interaction with professional phagocytes. The lab also work rationally applying these knowledge to generate an effective particle vaccine. Clinical research involves a project where nanomaterials and dendritic cells are used to boost immune responses against malignant tumors in dogs (i.e., personalized immunotherapy). Teaching interest include application of modern instructional methodologies to teach Veterinary Clinical Pathology
- Assistant Professor of Veterinary Clinical Pathology, Department of Veterinary Clinical Sciences, Washington State University, College of Veterinary Medicine, WA, USA, Jan 2013 to 2019 .http://www.wsu.edu/
- Assistant Professor of Pathology, Universidade de Fortaleza, School of Medicine, CE, Brazil, Jan 2009 to Dec 2012. http://www.unifor.br/
- Pathology Lecturer, Faculdade UniChristus, CE, Brazil, Sep 2009 to Dec 2012. http://unichristus.edu.br/
- Pathology Lecturer, Department of Pathology and Legal Medicine, Universidade Federal do Ceara, School of Medicine and Dentistry, CE, Brazil, Jan 2010 to Dec 2012. http://www.medicina.ufc.br/
- Veterinary Clinical Pathologist, Laforvet, Jan 2009 to Dec 2012. http://www.laforvet.com.br/
- Assistant Professor of Veterinary Clinical Pathology, Faculty of Veterinary Medicine, Universidade de Passo Fundo, RS, Brazil, Jun 2002 to Sept 2003. http://www.upf.br
- Abdellrazeq GS, Elnaggar MM, Bannantine JP, Schneider DA, Souza CD, Hwang J, Mahmoud AHA, Hulubei V, Fry LM, Park KT, Davis WC. (2019) A peptide-based vaccine for Mycobacterium avium subspecies paratuberculosis. Vaccine pii: S0264-410X(19)30504-3. doi: 10.1016/j.vaccine.2019.04.040. [Epub ahead of print] PMID: 31003915
- Abdellrazeq GS, Elnaggar MM, Bannantine JP, Park KT, Souza CD, Backer B, Hulubei V, Fry LM, Khaliel SA, Torky HA, Schneider DA, Davis WC. (2018) A Mycobacterium avium subsp. paratuberculosis relA deletion mutant and a 35 kDa major membrane protein elicit development of cytotoxic T lymphocytes with ability to kill intracellular bacteria. Vet Res. 49(1):53. doi: 10.1186/s13567-018-0549-3. PMID: 29941017
- Souza, CD, Bannantine, JP, Brown, WC, Davis, WC, et al. (2017) A nano particle vector containing recombinant Mycobacterium avium subsp paratuberculosis peptides stimulate a pro-immune profile in bovine macrophages. Journal of Applied Microbiology. doi: 10.1111/jam.13491.
- D. Guldner, P. Ziaei, Maria C Cardieri, Meaghan Eren, M. Grant Norton, Souza. CD (corresponding author). (2016) In vitro evaluation of the biological responses of canine macrophages challenged with PLGA nanoparticles containing Monophosphoryl Lipid A. PLoS One 11(11).
- Souza CD. (2015) Blocking the mitogen activated protein kinase-p38 pathway is associate with increase expression of Nitric Oxide Synthase and higher production of Nitric Oxide by bovine macrophages infected with Mycobacterium avium subsp paratuberculosis. Veterinary Immunology and immunopathology. doi:10.1016/j.vetimm.2015.01.007.
- Bannantine, J., Stable, J., Laws, E., Cardieri, MC., Souza CD (corresponding author). (2015) Mycobacterium avium subspecies paratuberculosis recombinant proteins modulate antimycobacterial functions of bovine macrophages. PLoS ONE 10(6): e0128966. doi:10.1371.
- Souza C, Davis WC, Eckstein TM, Sreevatsan S, Weiss DJ (2013) Mannosylated lipoarabinomannans from Mycobacterium avium subsp. paratuberculosis alters the inflammatory response by bovine macrophages and suppresses killing of Mycobacterium avium subsp. avium organisms. PLoS One 8(9) PMID: 24098744 PMCID: PMC3786972
- Weiss, DJ and Souza, CD. (2008) Modulation of Macrophage Function by Mycobacterium avium subsp. paratuberculosis: A Review. Veterinary Pathology. 45:829-841.
- Souza, CD; Weiss, DJ, Evanson, O. (2008) Cell membrane receptors involved in suppression of monocyte anti-microbial activity against Mycobacterium paratuberculosis. Microbial Pathogenesis. 44(3):215-23.
- Weiss, DJ,;Souza, CD; Evanson; O. Sanders; M, Rutherford. (2008) Bovine monocyte TLR2 receptors differentially regulate the intracellular fate of Mycobacterium avium subsp. paratuberculosis and Mycobacterium avium subsp. avium. Journal of Leukocyte Biology. 83(1):48-55.
- Weiss, DJ; Souza, CD; Evanson, O. (2008) Nuclear factor-kB regulates cytokine expression and apoptosis in bovine monocytes infected with Mycobacterium avium subsp. paratuberculosis. American Journal of Veterinary Research 69(6):804-10.
- Souza, CD; Weiss, DJ, Evanson, O. (2007) Role of the mitogen-activated protein kinase pathway in the differential response of bovine monocytes to Mycobacterium avium subsp. paratuberculosis and Mycobacterium avium subsp. avium. Microbes and Infection. 9(14-15):1545-52.
- Souza, CD; Weiss, DJ, Evanson, O. (2007) Role of the MAPK-ERK pathway regulating cytokine expression by Mycobacterium avium subsp. paratuberculosis-infected bovine monocytes. American Journal of Veterinary Research. 68(6):625-30.
- Souza, CD; et al. (2007) Cell Membrane Receptors in Bovine Mononuclear Phagocytes Involved in Phagocytosis of Mycobacterium avium subsp. paratuberculosis. American Journal of Veterinary Research. (68): 975-980.
- Souza, CD; Weiss, DJ, Evanson, O. (2006) Mitogen Activated Protein Kinase-p38 pathway is an important component of the anti-inflammatory response in Mycobacterium avium-infected bovine monocytes. Microbial Pathogenesis. v. 41(2-3):59-66.
- Weiss, DJ, Souza, CD, Evanson, O. (2006) Mucosal immune response in cattle with, subclinical Johne's disease. Veterinary Pathology,. (43): 127-135.
- Souza, CD; Weiss, DJ, Evanson, O. (2006) Jun n-terminal kinase (MAPK-JNK/SAPK) regulates cytokine expression in Mycobacterium avium subsp. paratuberculosis-infected bovine monocytes. American Journal of Veterinary Research Vol. 67, No. 10: 1760-1765.
- Souza, CD; Lopes, STA, Batina, P, et al. (2006) Parasitic stress in Saanen Goats: Evaluation of the CBC and neutrophilic oxidative burst. Revista Veterinaria Noticias. v. 12, n. 2, p. 35-40.
- Weiss, DJ, Evanson, O, Souza, CD. (2005) Expression of interleukin-10 and suppressor of cytokine signaling-3 associated with susceptibility to Jonhe's disease. American Journal of Veterinary Research. 66 (7):1114-20.